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01121 Journal of Nara Medical Association >
Vol.45 No.1 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/1749

Title: Pachytene期精母細胞の超微構造と免疫電顕的検討 : 減数分裂前S期の染色体複製とシナプトネマ構造
Other Titles: IMMUNOELECTRON MICROSCOPIC AND ULTRASTRUCTURAL STUDY OF RAT PACHYTENE SPERMATOCYTE : CHROMOSOME REPLICATION IN PRE-MEIOTIC INTERPHASE AND SYNAPTONEMAL COMPLEXES
Authors: 西野, 俊彦
Keywords: rat testis
synaptonemal complexes
chromatin radial loop
unclear skeleton
bromodeoxyuridin (BrdU)
chromosome replication
Issue Date: 28-Feb-1994
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.45 No.1 p.77-88
Abstract: The synaptonemal complexes (SCs), the unique organelle of the meiotic prophase nucleus, play important roles in the meiosis-specific events of homologous chromosome pairing, genetic recombination, and chromosome disjunction. Recently the SCs structure has been actively studied using immunohistochemical technique. However, it is still unknown whether nuclear skeleton in premeiotic interphase basically corresponds with SCs in meiotic prophase in spite of the resemblance of the shapes in these two structures. Therefore, I studied the meiotic prophase nucleus in rat testis after hypotonic treatment under the electron microscopy. Moreover, the male rat was infused continuously with a low dose bromodeoxyuridine (BrdU) from the tail vein, following a modified Counce-Meyer's microspreading procedure and immunogold labeling was performed in this study. The results were as following : (1) The SCs are composed of at least two different electron dense granules and chromatin fibers. These granules and fibers form a radial loop in lateral or central elements as hitherto assumed. (2) The replication patterns of autosomal chromatin were similar to those of sex chromatin. (3) Chromosome replications had a time lag between cross of SCs part and near the SCs part, indicating that SCs and nuclear skeleton is different structure organelle. These results suggest that continuous infusion of low dose BrdU is useful for DNA replication study.
URI: http://hdl.handle.net/10564/1749
ISSN: 04695550
13450069
Appears in Collections:Vol.45 No.1

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