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01121 Journal of Nara Medical Association >
Vol.45 No.2 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/1716

Title: 胆汁酸の肝細胞膜輸送に及ぼすグルカゴンの影響
Authors: 久保, 良一
Keywords: glucagon
bile acid
hepatocellular membrane transport
Issue Date: 30-Apr-1994
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.45 No.2 p.228-235
Abstract: We have found that serum total bile acid (TBA) levels decreased in some patients with liver cirrhosis after intravenous administration of 1 mg glucagon. In the present study, the mechanism of this phenomenon was investigated in vitro using the hepatocyte culture system. The effect of the addition of glucagon on the membrane transport of ¹⁴C-taurocholic acid (¹⁴C-TCA) was studied in freshly isolated rat hepatocytes. The results were as follows : 1) Glucagon distinctly stimulated the release of cyclic AMP from hepatocytes in the standard medium. 2) Glucagon enhanced ¹⁴C-TCA uptake by hepatocytes in a concentration-dependent manner between 10⁻⁷~10⁻⁵ mol/L. 3) Dibutyryl cyclic AMP also enhanced the ¹⁴C-TCA uptake at the concentration of 10⁻⁵ or 2×10⁻⁵ mol/L. 4) The uptake curves of ¹⁴C-TCA by hepatocytes both with and without glucagon (10⁻⁶ mol/L) were linear until 120 seconds and reached a constant value by 900 seconds in the standard medium. However, in the Na⁺⁻free medium or in the ouabain-added standard medium, the uptakes were strongly depressed either with or without an addition of glucagon. 5) In a kinetic analysis, the hepatocytes uptake of ¹⁴C-TCA either with or without glucagon showed a saturation curve of the Michaelis-Menten type. With an addition of glucagon Vmax was increased, although the Km was unchanged. In conclusion, glucagon promoted the Na⁺⁻dependent active carrier-mediated transport in hepatocytes and increased their bile acids uptake.
URI: http://hdl.handle.net/10564/1716
ISSN: 04695550
Appears in Collections:Vol.45 No.2

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