旋毛虫症に特異的な免疫診断法に関する研究

Abstract

Trichinella spiralis muscle larvae possessed both "specific" antigens in stichocyte α-granules, the alimentary tract, and the cuticle surface ; and "non-specific" antigens in cuticle inner layer(s), hypodermis, and hemolymph. Thin study was designed to determine immunocytochemically the class of antibodies raised against specific and non-specific antigens. Ultrathin sections of resin embedded T. spiralis muscle larvae were immunostained with sera from patients with a wide variety of helminthiasis including trichinosis, paragonimiasis, fascioliasis, trichuriasis, gnathostomiasis, dirofilariasis, anisakiasis, cysticercosis cellulosae hominis, and diphyllobothriasis. Sera from all patients reacted positively against the non-specific antigens. IgG-class antibodies exclusively contributed to the response. On the other hand, patients with trichinosis evoked both IgG- and IgM-class antibodies against specific and non-specific antigens. These results increase the potential usefulness of the specific diagnosis of trichinosis, because they suggest that a test using only IgM-class antibodies may minimize the undesirable effects of contaminating non-specific antigens in the immunodiagnosis of trichinosis.