呼吸器疾患におけるchemiluminescence法を用いた好中球機能の検討

Abstract

The functions of neutrophilis in the peripheral venous blood of patients with respiratory infection or with lung cancer were assayed by measuring the chemiluminescent response elicited by the cells. A total of 57 cases was subject to chemiluminescence (CL) assay : 37 cases of acute respiratory infection, 5 at the stable phase of chronic lower tract infection, and 15 of lung cancer. CL was evaluated by CL-index (patients' CL/controls' CL). In acute respiratory infection, the neutrophil CL-index・N (CL-index × neutrophil numbers) was decreased by antimicrobial chemotherapy, in association with improvement of their clinical course. Neutrophil CL-index・N was correlated with the whole blood CL-index in 12 cases in which the whole blood CL was assayed. On the other hand, at the stable phase of chronic lower tract infection, neither neutrophil CL-index nor the neutrophil CL-index・N changed remarkably after antimicrobial chemotherapy. In cases of lung cancer, whole blood CL fell as the number of neutrophils was reduced by anticancer chemotherapy. Granulocyte colony stimulating factor (G-CSF) treatment increased the whole and neutrophil CLs. Early initiation of G-CSF treatment resulted in a rapid increase in CL values of the whole blood and isolated neutrophils. These results suggest that measurement of CL is a useful assay for neutrophil function in respiratory disease.