マウス補体第一成分の亜成分C1qに関する研究 ： ＩgG2b産生ハイブリドーマ移入によるC1q代謝への影響の解析

Abstract

Serum C1q levels and C1q mRNA from peritoneal exudate cells and spleen cells were measured in BALB/c female mice treated intraperitoneally with IgG 2b hybridoma cells (2×10⁶/0.2 ml) and/or pristane, as well as in untreated mice. In the hybridoma treated mice, both C1q and IgG levels were significantly increased compared with pristane treated mice and/or untreated mice. Northern blot analysis of total RNA from peritoneal exudate cells and spleen cells of these mice showed one band of approximately 1.2 kb. The highest signal was found in RNA preparations of hybridoma treated peritoneal macrophage. These results suggest that there may be some anabolic as well as catabolic interaction between C1q and IgG 2b syntheses.