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Vol.45 No.6 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/1608

Title: 糖尿病性腎症における尿中糖蛋白分析 : ウエスタンブロッティング分析によるレクチン結合パターン
Other Titles: ANALYSIS OF URINARY GLYCOPROTEINS FOR DIABETIC NEPHROPATHY : WESTERN BLOTTING ANALYSIS USING PEROXIDASE LABELED LECTINS
Authors: 中島, 靖夫
Keywords: diabetic nephropathy
electrophoretic analysis
high performance lectin affinity chromatography
lectin
Issue Date: 31-Dec-1994
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.45 No.6 p.672-683
Abstract: The aim of this study was to evaluate the clinical usefulness of polyacrylamide gel electrophoresis,followed by reaction with peroxidase-coupled lectins using urinary glycoproteins in 20 patients with diabetic nephropathy. After SDS-polyacrylamide gel electrophoresis (SDS-PAGE),high performance lectin affinity chromatography (HPLAC) and immunoblotting of urinary glycoproteins with α₁-acid glycoprotein (α₁-AG) and α₁-microglobulin (α₁-MG) were performed. The lectins used were derived from Triticum vulgaris (WGA),Phaseolus vulgaris (PHA-E₄),Dolichos biflorus (DBA) and Lens culinaris (LCA),which had a high affinity for β1→4 N-acetyl-D-gulcosamine (GlcNAcβ1→4GlcNAc),N-acetyl-D-galactosamine (GalNAc),α-galactosamine (α-GalNAc) and α-mannose (α-Man) residues,respectively. Electrophoretic patterns of urinary glycoproteins clearly showed the presence of lectin-reactive glycoproteins with molecular weights lower than that of albumin. The molecular weights of the main bands that reacted with WGA,namely PHA-E₄ and LCA,were respectively 50,000 and 38,000 daltons,and increased with the progression of diabetic nephropathy. WGA reacted strongly with many glycoproteins having a wide range of molecular weights. LCA and PHA-E₄ reacted preferentially with glycoproteins of molecular weight lower than 50,000 daltons,but with DBA no reaction was observed. These results suggest that low molecular-weight glycoproteins that are observed in the urine of patients with diabetic nephropathy have abundant carbohydrate residues such as GlcNAc β1→4 GlcNAc,GalNAc and α-Man,and include α₁-AG and α₁-MG. The excretion of low-molecular-weight glycoproteins with high affinities for some lectins suggests organic impairment in diabetic nephropathy.
URI: http://hdl.handle.net/10564/1608
ISSN: 04695550
13450069
Appears in Collections:Vol.45 No.6

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