DSpace コレクション: 1994-04
http://hdl.handle.net/10564/1696
1994-042024-03-29T05:21:56Z実験的骨形成モデルを用いた初期の骨形成に及ぼすカドミウムの影響 : アルカリフォスファターゼ,カルシウム,燐,およびオステオカルシンmRNAの動態
http://hdl.handle.net/10564/1720
タイトル: 実験的骨形成モデルを用いた初期の骨形成に及ぼすカドミウムの影響 : アルカリフォスファターゼ,カルシウム,燐,およびオステオカルシンmRNAの動態
著者: 勝田, 敏哉
抄録: To determine the direct effect of cadmium (Cd) on bone formation, the potential of Cd-treated bone marrow cells and demineralized bone matrix (DBM) to form bone and cartilage was assessed using a diffusion chamber (DC) in vivo, by measurement of biochemical parameters such as alkaline phosphatase (ALP) activity, total calcium and phosphorus contents, the bone-specific protein, osteocalcin content, and by the gene expression of osteopontin and osteocalcin. Diffusion chambers were inoculated with DBM and bone marrow cells from either Cd-treated or non-treated rats (control) and were then implanted subcutaneously into syngeneic non-treated rats. Unlikely in control DC, a peak of ALP activity did not occur at 4 weeks postimplantation in DC implants inoculated with Cd-treated bone marrow. ALP activity, and calcium and phosphorus contents in these Cd-treated DC implants were significantly lower than those of the control DCs at the early stage of implantation. The accumulation of osteocalcin in DCs with Cd-treated bone marrow was also significantly
lower than that in control DCs. By gene expression analyses, osteopontin mRNA was intensly expressed in the DC with control and followed with Cd-treated bone marrow DC 5 weeks after implantation. On the contrary, the expression of osteocalcin mRNA in the DCs with Cd-treated bone marrow was much lower than that in control DCs by both Northern blot analysis and also in situ
hybridization. These results indicate that Cd administration restrains the osteoblastic
differentiation pathway in bone marrow through direct effects on these cells.1994-04-29T15:00:00Z多種類の市販感冒剤服用による一中毒死例
http://hdl.handle.net/10564/1718
タイトル: 多種類の市販感冒剤服用による一中毒死例
著者: 森村, 佳史; 河原, 信吾; 石谷, 昭子; 伊藤, 信彰; 平野, 佳成; 内田, 和人; 東, 裕子; 廣田, 忠臣
抄録: A 22-year-old woman was found dead in her locked room. She was suspected to have ingested large amounts of 5 kinds of commercial cold medicine containing varying combinations and contents of analgesic and antipyretic drugs, central stimulants, antitussives, and antihistamines. Drugs were extracted from blood, urine, liver, kidney, brains, and contents of the stomach, and qualitative and quantitative estimation of these drugs was carried out by GC and GC-MS. The concentration (μg/ml) of acetaminophen, dihydrocodein-phosphate, dl-methylephedrine-hydrochloride, caffein, and noscapine in the
heart blood were 880, 6, 23, 70, and 114, respectively. The concentrations of acetaminophen, dihydrocodein-phosphate and dl-methyl-ephedrine are high enough to be lethal. Autopsy revealed marked hyperemia in several organs, especially in the lung and kidney. Histological examination of various organs and tissues showed remarkable hyperemia and bleeding in the lung, vacuolation of hepatic cells and hepatic necrosis around the central vein, and necrosis of kidney tubules. These histological findings correspond well to those observed in acetaminophen intoxication. The overdose of acetaminophen, dihydrocodein-phosphate and dl-methyl-ephedrine are supposed to be the cause of death.1994-04-29T15:00:00ZMethicillin Resistant Staphylococcus aureus (MRSA)に対するBifidobacterium breveの抗菌活性と活性物質の精製
http://hdl.handle.net/10564/1717
タイトル: Methicillin Resistant Staphylococcus aureus (MRSA)に対するBifidobacterium breveの抗菌活性と活性物質の精製
著者: 井上, 敦子
抄録: The staphylococci, known to be members of the normal flora of the nose, are sometimes involved in chronic sinusitis. Recently, non-chemotherapeutic approaches have assumed importance in the treatment of chronic staphylococcal infections, since the rapid emergence of resistance to commonly-employed antimicrobial drugs has made it difficult to eradicate resistant staphylococci such as a methicillin resistant Staphylococcus aureus (MRSA) from the lesion. Thus, the present study was done to show the antimicrobial
activity of Bifidobacterium breve to MRSA isolated from a patient with chronic sinusitis. Using MRSA resistant to acidic condition, MRSA could not survive after day 1 in the culture supernatant of B. breve, but in the control of 0.01 M phosphate-buffered saline containing tryptic soy broth of which pH was adjusted to 4.3, growth of MRSA was not affected. This anti-MRSA activity by the culture supernatants of B. breve is active only in the acidic condition under pH 4.5. But using tryptic soy soft agar containing 1% lactose, the activity was effective at pH 7.0. Purification of the active moiety from the culture supernatant of B. breve was done with ion exchange chromatography. Investigation of the main ingredient of the active moiety was done with the Anthron, Lowry, and Orcinol methods, and the absorption spectrum was calculated. The main component of the active
moiety is considered to contain nucleotide and the molecular weight of the moiety will be around 8000. Our study is in progress to isolate the active moiety exhibiting anti-MRSA activity. Therefore it may be possible to apply this anti-MRSA activity of B. breve for clinical use in future.1994-04-29T15:00:00Z胆汁酸の肝細胞膜輸送に及ぼすグルカゴンの影響
http://hdl.handle.net/10564/1716
タイトル: 胆汁酸の肝細胞膜輸送に及ぼすグルカゴンの影響
著者: 久保, 良一
抄録: We have found that serum total bile acid (TBA) levels decreased in some patients with liver cirrhosis after intravenous administration of 1 mg glucagon. In the present study, the mechanism of this phenomenon was investigated in vitro using the hepatocyte culture system. The effect of the addition of glucagon on the membrane transport of ¹⁴C-taurocholic acid (¹⁴C-TCA) was studied in freshly isolated rat hepatocytes. The results were as follows : 1) Glucagon distinctly stimulated the release of cyclic AMP from hepatocytes in the standard medium. 2) Glucagon enhanced ¹⁴C-TCA uptake by hepatocytes in a concentration-dependent manner between 10⁻⁷~10⁻⁵ mol/L. 3) Dibutyryl cyclic AMP also enhanced the ¹⁴C-TCA uptake at the concentration of 10⁻⁵ or 2×10⁻⁵ mol/L. 4) The uptake curves of ¹⁴C-TCA by hepatocytes both with and without glucagon (10⁻⁶ mol/L) were linear until 120 seconds and reached a constant value by 900 seconds in the standard medium. However, in the Na⁺⁻free medium or in the ouabain-added standard medium, the uptakes were strongly depressed either with or without an addition of glucagon. 5) In a kinetic analysis, the hepatocytes uptake of ¹⁴C-TCA either with or without glucagon showed a saturation curve of the Michaelis-Menten type. With an addition of glucagon Vmax was increased, although the Km was unchanged. In conclusion, glucagon promoted the Na⁺⁻dependent active carrier-mediated transport in hepatocytes and increased their bile acids uptake.1994-04-29T15:00:00Z