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oai:ginmu.naramed-u.ac.jp:10564/18712017-06-11T23:20:26Zhdl_10564_1863表紙、目次、投稿規程、奥付(Vol.62 No.3,4,5)表紙目次投稿規定奥付奈良医学会奈良県立医科大学OtherOthersapplication/pdfJournal of Nara Medical Association Vol.62 No.3,4,513450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1871Journal of Nara Medical Association, 62(3,4,5)http://hdl.handle.net/10564/1871http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1871/1/00p.%e8%a1%a8%e7%b4%99%e3%80%81%e7%9b%ae%e6%ac%a1%e3%80%81%e6%8a%95%e7%a8%bf%e8%a6%8f%e7%a8%8b%e3%80%81%e5%a5%a5%e4%bb%98%28Vol.62_No.3%2c4%2c5%29.pdf13450069AA11252383Journal of Nara Medical Association623,4,52011-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18742020-08-18T02:16:48Zhdl_10564_1863Multi-color / target fluorescence in situ hybridizatio(Mt -FISH)法及び免疫染色(ICC)法を用いた腹腔細胞診の検討THE UTILITY OF MULTI-COLOR/TARGET FLUORESCENCE (Mt-FISH) in situ HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY (ICC) METHODS IN PERITONEAL CYTOLOGY榎本, 泰典笠井, 孝彦武田, 麻衣子高野, 将人森田, 剛平柳生, 貴裕嶋田, 亜也森川, 佐和子西川, 武peritoneal cytologMulti-color/target fluorescence in situ hybridization(Mt-FISH)immunocytochemistry (ICC)Introduction: Utility of Mt-FISH analysis in peritoneal cytology has not been well elucidated so far. In the present study,therefore,peritoneal cytology in 63 patients with malignant and non-malignant diseases was studied in different three methods,Papanicolaou and Giemza cytology (Pap),ICC,and Mt-FISH,to evaluate the utility and availability of ICC and Mt-FISH in peritoneal cytology.
Materials and methods: Peritoneal cytology specimens of 63 patients were collected
from the files of the Pathology Section,Nara Medical University Hospital,including 54 cases of adenocarcinoma (33 of gastrointestinal or biliary, 18 ovarian or endometrial, and 3 primary peritoneal cancers) and 9 cases of benign lesions (6 benign tumors and 3 non-tumor lesions). Results of the three different methods were evaluated and compared.
Results: Incidence of positive malignancy rate was 20% (11/54) in Pap method,36%
(18/50) in ICC,and 59% (20/34) in Mt-FISH. When compared to Pap method, sensitivity
and accuracy were statistically significantly higher in Mt-FISH, but not in ICC. No inadequate or insufficient specimens were found in Pap method, but these were found in 6% of lavage cytology and 8% of ascitic cytology in ICC specimens and 14% of lavage cytology and 31% of ascitic cytology in Mt-FISH specimens, respectively.
Conclusion: ICC and Mt-FISH methods are very useful additional methods for accurate
diagnosis of peritoneal cytology. But the incidence of inadequate or insufficient cytology specimens was higher in Mt-FISH of ascitic cytology, and the methods of processing the ascitic fluid for Mt-FISH must be improved and are now under consideration.奈良医学会奈良県立医科大学ArticleDepartmental Bulletin Paperapplication/pdfJournal of Nara Medical Association Vol.62 No.3,4,5 p.49-5413450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1874Journal of Nara Medical Association, 62(3,4,5): 49-54http://hdl.handle.net/10564/1874http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1874/1/49-54p..pdf13450069AA11252383Journal of Nara Medical Association623,4,549542011-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18752017-05-29T06:07:56Zhdl_10564_1863PLASMA CELL INFILTRATION AND MUCOID DEGENERATION IN THE MEDIA OF ASCENDING AORTA IN PATIENT WITH CORONARY ARTERY DISEASENagareda, TomofumiHirata, NobuakiKosakai, YoshioOhsawa, Masahikoaortic mediaatherosclerosiscoronary artery diseasemucoid degenerationplasma cell infiltrationAims Atherosclerosis results in inflammatory changes in the aortic intima,
but little is known regarding medial changes. Atherosclerosis of the ascending aorta
coexists with coronary artery disease. The aim of this study was to investigate the
atherosclerotic changes in 44 biopsy specimens of media of the ascending aorta
associated with coronary artery disease. Plasma cells do not appear in non-inflammatory
tissue.
Methods We compared plasma cells,and matrix metalloproteinase (MMP)-2-,-9- and
-12-positive cells immunohistochemically,and we also compared mucoid degeneration
and fibrosis determined by staining using a point-counting method,for groups with a
variable number of coronary stenotic (≧75%)lesions.
Results In patients with one to three coronary stenotic lesions,plasma cells and
mucoid degeneration were low in the aortic media. With four to five lesions,bo th plasma
cells and mucoid degeneration increased significantly compared with those in the group
with one to three lesions,and MMP-12-positive cells significantly decreased. In patients
with six to nine lesions,the number of plasma cells was significantly lower than in
patients with four or five lesions,whereas mucoid degeneration significantly increased.
There was no change in fibrosis.
Conclusions These findings may help us to better understand and treat atherosclerosis.奈良医学会奈良県立医科大学ArticleDepartmental Bulletin Paperapplication/pdfJournal of Nara Medical Association Vol.62 No.3,4,5 p.55-6713450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1875Journal of Nara Medical Association, 62(3,4,5): 55-67http://hdl.handle.net/10564/1875http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1875/1/55-67p..pdf13450069AA11252383Journal of Nara Medical Association623,4,555672011-10-31engpublisher
oai:ginmu.naramed-u.ac.jp:10564/18762021-05-25T01:53:03Zhdl_10564_1863MOLECULAR DETECTION OF MECT1-MAML2 FUSION GENE IN MUCOEPIDERMOID CARCINOMA WITH ORDINARY AND VARIANT HISTOLOGY: A STUDY USING ARCHIVAL PARAFFIN EMBEDDED TISSUEKasai, TakahikoTakeda, MaikoEnomoto, YasunoriTakano, MasatoMorita, kouheiNonomura, Akitakamucoepidermoid carcinomaMECT1-MAML2 fusion geneRT-PCRparaffin-embedded tissueMucoepidermoid carcinoma (MEC) has been characterized by t (11; 19)(q21;
p13). This chromosomal translocation has been recently shown to generate a MECT1-
MAML2 fusion gene. MEC can pose diagnostic challenges when they are of high-grade,
of variant histologic appearance and occurring in an unusual site. The aim of this study
was to evaluate the frequency of the MECT1-MAML2 fusion gene among primary
salivary gland MECs and extrasalivary gland MECs, together with some histological
variants and its role as a possible diagnostic adjunct, comparing the salivary gland
tumors including Warthin's tumor(WT),pleomorphic adenoma(PA),and adenoid cystic
carcinoma(ACC). Using a reverse transcription-polymerase chain (RT-PCR)-based
approach, we assayed for the MECT1-MAML2 transcript in 39 cases for which paraffin-
embedded tumor tissue with adequate RNA was available. These included 19 MECs,10
WTs, five PAs, and five ACCs. The MECT1-MAML2 fusion gene transcript was
detected in 16 (84.2%) of 19 MECs. These positive cases included two cases of MEC with
WT-like areas,a sclerosing MEC and a clear cell MEC. Three negative cases were high-
grade MECs. Two of them were not easy to distinguish from squamous cell carcinoma.
The MECT1-MAML2 fusion gene was negative in all cases of WT,PA and ACC. The
potential usefulness of MECT1-MAML2 fusion gene expression as a molecular marker in
the diagnosis of MEC is supported.奈良医学会奈良県立医科大学ArticleDepartmental Bulletin Paperapplication/pdfJournal of Nara Medical Association Vol.62 No.3,4,5 p.69-7913450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1876Journal of Nara Medical Association, 62(3,4,5): 69-79http://hdl.handle.net/10564/1876http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1876/1/69-79p..pdf13450069AA11252383Journal of Nara Medical Association623,4,569792011-10-31engpublisher
oai:ginmu.naramed-u.ac.jp:10564/18772017-05-29T06:07:57Zhdl_10564_1863AN AUTOPSY CASE OF PORTOPULMONARY HYPERTENSION ASSOCIATED WITH ALCOHOLIC LIVER CIRRHOSISMaruyama, HiroshiToyoda-Hirana, YukiNakamura, TakahitoEnomoto, YasunoriHamada, KaoruKimura, Hiroshiportopulmonary hypertensioa lcoholic liver cirrhosisp lexiform lesionWe report an autopsy case of pulmonary pexogenic arteriopathy associated with portal hypertension due to alcoholic liver cirrhosis, termed portopulmonary hypertension (PPHT). A 49-year-old man who has had alcoholic liver cirrhosis for 10 years complained of severe dyspnea (Fletcher-Hugh-Jones V). Chest CT revealed marked
enlargement of bilateral hilar pulmonary arteries and cardiomegaly associated with right ventricular hypertrophy. The patient died from hepatic. encephalopathy and respiratory failure. Autopsy c1early revealed the wall thickness of pulmonary small vessels diffusely in peripheral fields on cut surfaces and marked dilatation of the main pulmonary artery, together with liver cirrhosis. Microscopically, the pulmonary small arteries demonstrated grade 5 pulmonary plexogenic arteriopathy inc1uding plexiform lesions and a micronodule resembling an arachnoid granulation or meningioma throughout the lungs. This case suggested that a typical plexogenic arteriopathy morphologically and definitely
contributed to confirm PPHT, although the patient was c1inically suspected of hepatopulmonary syndrome (HPS).奈良医学会奈良県立医科大学Departmental Bulletin Paperapplication/pdfJournal of Nara Medical Association Vol.62 No.3,4,5 p.81-87http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1877Journal of Nara Medical Association, 62(3,4,5): 81-87http://hdl.handle.net/10564/1877http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1877/1/81-87p..pdf13450069AA11252383Journal of Nara Medical Association623,4,581872011-10-31engpublisher
oai:ginmu.naramed-u.ac.jp:10564/18782017-05-29T06:07:49Zhdl_10564_1863第131回奈良医学会 : 学会記事第131回奈良医学会学会記事奈良医学会奈良県立医科大学ArticleDepartmental Bulletin Paperapplication/pdfJournal of Nara Medical Association Vol.62 No.3,4,5 p.89-9313450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1878Journal of Nara Medical Association, 62(3,4,5): 89-93http://hdl.handle.net/10564/1878http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1878/1/89-93p..pdf13450069AA11252383Journal of Nara Medical Association623,4,589932011-10-31jpnpublisher