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oai:ginmu.naramed-u.ac.jp:10564/18592017-06-11T23:20:26Zhdl_10564_1858表紙、目次、投稿規定、奥付(Vol.43 No.5)表紙目次投稿規定奥付奈良医学会OtherOthersapplication/pdf奈良医学雑誌 Vol.43 No.50469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1859奈良医学雑誌, 43(5)http://hdl.handle.net/10564/1859http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1859/1/%e8%a1%a8%e7%b4%99%e3%80%81%e7%9b%ae%e6%ac%a1%e3%80%81%e6%8a%95%e7%a8%bf%e8%a6%8f%e5%ae%9a%e3%80%81%e5%a5%a5%e4%bb%98%28Vol.43_No.5%29.pdf04695550AN00180974奈良医学雑誌4351992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18602017-05-29T06:07:50Zhdl_10564_1858奈良県下ではじめて確認されたつつが虫病の1例FIRST CASE OF TSUTSUGAMUSHI DISEASE IN NARA PREFECTURE喜多, 公雄石井, 禎暢森安, 博人松山, 義則西山, 利正荒木, 恒治福井, 博辻井, 正tsutsugamushi diseaseNara PrefectureA 58-year-old farmer in Higashiyoshino, Nara Prefecture, was admitted to this hospital with high fever and general rash on Aug. 15, 1990. There was an eschar on the left arm and a tender swelling of the left axillary lymph node. Peripheral blood revealed leukopenia and thrombocytopenia. Blood chemistry showed marked elevation of LDH and
CPK. From these clinical findings tsutsugamushi disease was suspected. The patient was treated with minocycline and recovered well. The diagnosis of tsutsugamushi disease was confirmed by indirect immunoperoxidase test. This is the first recorded case of tsutsugamushi disease in Nara Prefecture.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.449-4520469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1860奈良医学雑誌, 43(5): 449-452http://hdl.handle.net/10564/1860http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1860/1/449-452p%ef%bc%9a%e5%a5%88%e8%89%af%e7%9c%8c%e4%b8%8b%e3%81%a7%e3%81%af%e3%81%98%e3%82%81%e3%81%a6%e7%a2%ba%e8%aa%8d%e3%81%95%e3%82%8c%e3%81%9f%e3%81%a4%e3%81%a4%e3%81%8c%e8%99%ab%e7%97%85%e3%81%ae1%e4%be%8b.pdf04695550AN00180974奈良医学雑誌4354494521992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18612017-06-11T23:20:26Zhdl_10564_1858全血凝固過程中のヒト第Ⅷ因子/von Willebrand因子複合体の動態CHANGES OF HUMAN FACTOR Ⅷ/VON WILLEBRAND FACTOR COMPLEX DURING WHOLE BLOOD CLOTTING藤原, 利治Factor Ⅷ activity (F Ⅷ : C)Factor Ⅷ antigen (F Ⅷ : Ag)von Willebrand Factor (vWF)F Ⅷ/vWF complexwhole blood coagulationChanges of factor Ⅷ(F Ⅷ), von Willebrand Factor (vWF) and FV Ⅷ/vWF complex during whole blood clotting were investigated. 1. Although F Ⅷ activity in normal sera was low (13.9±12.0 u/dl), F Ⅷ antigen (F Ⅷ : Ag) was nearly normal (73.7±32.7 u/dl). Levels of vWF antigen (vWF : Ag) in the plasmas or sera assayed by immunoradiometric assay (IRMA) were 103.7±35.0 u/dl and 51.7±26.2 u/dl, respectively. Each value of F Ⅷ/vWF complex in the plasmas or sera
was 101.7±29.1 u/dl or 24.1±18.1 u/dl. 2. When platelet-rich plasma from normal individual was clotted with an equal volume
of 1/40 M CaCl_2, F Ⅷ showed a moderate decrease. Incubation of normal plasma with
various concentrations of phospholipid (PL) resulted in a dose-dependent loss of F Ⅷ : Ag. 3. In normal subjects, F Ⅷ : C increased 2-folds within 10 min after clot formation, then gradually decreased remaining the level around 10-20 u/dl. F Ⅷ : Ag also increased once, then decreased keeping the level around 50-60 u/dl. VWF : Ag assayed by IRMA increased once, then gradually decreased and stayed around 60-70 u/dl. F Ⅷ/vWF complex decreased rapidly after initiation of clotting, then it stayed at constant level of 10 u/dl at 20 min. 4. Time-course of F Ⅷ proteolysis during whole blood clotting was monitored by an enzyme-linked immunosorbent assay (ELISA). Interval between 30 and 60 min after coagulation, levels of the aminoterminal "acidic" region of F Ⅷ light chain and of the middle "acidic" region of F Ⅷ heavy chain decreased to the same level of F Ⅷ : C. However, the 72 kDa-fragment of F Ⅷ light chain decreased more slowly. These results indicate that F Ⅷ is immediately dissociated from F Ⅷ/vWF complex after initiation of whole blood clotting, and the F Ⅷ undergoes rapid proteolytic modification
in serum.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.453-4630469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1861奈良医学雑誌, 43(5): 453-463http://hdl.handle.net/10564/1861http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1861/1/453-463p%ef%bc%9a%e5%85%a8%e8%a1%80%e5%87%9d%e5%9b%ba%e9%81%8e%e7%a8%8b%e4%b8%ad%e3%81%ae%e3%83%92%e3%83%88%e7%ac%ac%e2%85%a7%e5%9b%a0%e5%ad%90von_Willebrand%e5%9b%a0%e5%ad%90%e8%a4%87%e5%90%88%e4%bd%93%e3%81%ae%e5%8b%95%e6%85%8b.pdf04695550AN00180974奈良医学雑誌4354534631992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18622017-05-29T06:07:49Zhdl_10564_1858ニコチンによる急性毒性と加齢の影響についてSTUDIES ON ACUTE TOXICITY OF NICOTINE AND AGING岡本, 雅人nicotinacute toxicitydopaminergic neuronsaginghepatic metabolismThe mechanisms in the brain for acute toxicity of nicotine were investigated
in rats. Age-related changes in acute toxicity of nicotine were also studied. An acute intraperitoneal (i. p.) or intracerebroventricular (i. c. v.) injection of nicotine intensified convulsions and death dose-dependently. But those convulsive responses induced by nicotine (i. c. v.) were more rapid and shorter than those induced by nicotine (i. p.). Acute toxic
effects of nicotine were dose-dependently antagonized by mecamylamine (i. p. or i. c. v.), but not by hexamethonium (i. p.). Nicotine (10 or 24.5 mg/kg, i. p.) significantly increased dopamine, 3, 4-dihydroxyphenylacetic acid and homovanillic acid in striatum. Furthermore, electrical lesion of striatum suppressed acute toxicities of nicotine. Pretreatment of SCH-23390, but not of haloperidol or sulpiride, blocked nicotine-induced convulsions and death. Pretreatment of phenobarbioal, diazepam or MK-801 also prevented acute toxicity of nicotine. Nicotine levels in the blood and cortex after an acute i. p. injection of nicotine (24.5 mg/kg) were significantly higher in old rats than in young rats. Hepatic metabolisms of nicotine
decreased age-relatedly. Also, nicotine (24.5 mg/kg, i. p.)-induced lethality tended to increase in old rats. On the other hand, nicotine (24.5 mg/kg, i. p.) did not change
monoamine levels in any brain regions in old rats. These results indicate that acute toxic effects of nicotine can be attributed to a central site. Acute toxicity of nicotine may be related to dopaminergic neurons, especially in the nigrostriatal system, as well as GABAergic and glutamatergic neurons. Furthermore, stimulation of D1 receptors in the central dopaminergic system may be concerned with the
propagation of acute toxic effects of nicotine. Also, acute toxicity of nicotine in old rats may be involved in two significant decreases, in hepatic metabolism of nicotine and in brain sensitivity to nicotine.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.464-4740469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1862奈良医学雑誌, 43(5): 464-474http://hdl.handle.net/10564/1862http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1862/1/464-474p%ef%bc%9a%e3%83%8b%e3%82%b3%e3%83%81%e3%83%b3%e3%81%ab%e3%82%88%e3%82%8b%e6%80%a5%e6%80%a7%e6%af%92%e6%80%a7%e3%81%a8%e5%8a%a0%e9%bd%a2%e3%81%ae%e5%bd%b1%e9%9f%bf%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6.pdf04695550AN00180974奈良医学雑誌4354644741992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18642017-06-11T23:20:26Zhdl_10564_1858活動性肺結核患者の末梢血単球のIL-1β,TNFα およびIL-6産生能と栄養障害との相互関連性の検討A STUDY OF INTERACTION BETWEEN INTERLEUKIN-1β, TUMOR NECROSIS FACTOR α,AND INTERLEUKIN-6 PRODUCTION BY PERIPHERAL BLOOD MONOCYTES AND NUTRITIONAL DISTURBANCE IN ACTIVE PULMONARY TUBERCULOSIS塚口, 勝彦pulmonary tuberculosisinterleukin-1βtumor necrosis factor αinterleukin-6nutritionNutritional disturbance has been empirically observed in patients with pulmonary tuberculosis. We have already demonstrated that malnutrition is frequent in these patients by scientific nutritional assessment, although the mechanism of this nutritional disturbance remains unclear. To elucidate this mechanism, I measured the production of interleukin-1β (IL-1β), tumor necrosis factor α (TNFα), and interleukin-6 (IL-6) by peripheral blood monocytes, and correlated them with nutritional parameters in those patients. Supernatants of monocyte cultures after 24 hours stimulation by lipopolysaccharide were analysed by enzyme liked immunosorbent assay for IL-1β, TNFα, and IL-6. In order to assess nutritional status I measured some visceral proteins (serum albumin, transferrin, prelbumin, retionl binding protein), plasma amino acids, and anthropometrics. The results are as follows. (1) Patients with pulmonary tuberculosis were confirmed to be malnourished in visceral proteins, plasma amino acids, and anthropometric indices. (2) In patients with moderate and mild nutritional depletion the production of IL-1β, TNFα, and IL-6 was significantly higher than in healthy controls, and correlated inversely with the nutritional parameters. (3) In patients with severe nutritional depletion the production of these cytokines was significantly lower than in healthy controls. The present study suggested an interaction between nutritional status and these cytokines : the hypermetabolic effects of IL-1β, TNFα, and IL-6 might induce nutritional disturbance in patients with pulmonary tuberculosis, and severe nutritional disturbance might in turn diminish the production of these cytokines.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.475-4880469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1864奈良医学雑誌, 43(5): 475-488http://hdl.handle.net/10564/1864http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1864/1/475-488p%ef%bc%9a%e6%b4%bb%e5%8b%95%e6%80%a7%e8%82%ba%e7%b5%90%e6%a0%b8%e6%82%a3%e8%80%85%e3%81%ae%e6%9c%ab%e6%a2%a2%e8%a1%80%e5%8d%98%e7%90%83%e3%81%aeIL-1%ce%b2%ef%bc%8cTNF%ce%b1_%e3%81%8a%e3%82%88%e3%81%b3IL-6%e7%94%a3%e7%94%9f%e8%83%bd%e3%81%a8%e6%a0%84%e9%a4%8a%e9%9a%9c%e5%ae%b3%e3%81%a8%e3%81%ae%e7%9b%b8%e4%ba%92%e9%96%a2%e9%80%a3%e6%80%a7%e3%81%ae%e6%a4%9c%e8%a8%8e.pdf04695550AN00180974奈良医学雑誌4354754881992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18652017-06-11T23:20:26Zhdl_10564_1858生後発育に伴う胎児ヘモグロビンγ鎖異型体組成の変化について : いわゆるA-F switchingに関連してCHANGES OF THE γ ISOFORM COMPOSITIONS IN FETAL HEMOGLOBIN DURING POSTNATAL DEVELOPMENT大賀, 好美γ chain isoformsgene expressionHb switchingHb Fzygosity of twinsAn efficient procedure was developed for the enrichment of Hb F (α₂ γ₂) in human adult hemolysate by an alkali denaturation-salting out. The method can be applied to small amounts of blood with less than 1% of Hb F, and provides a sufficient Hb F level to allow an accurate high-performance liquid chromatographic (HPLC) quantitation of the three γ chain isoforms, ᴳγ (136 Gly, 75 Ile), ᴬγᴵ (136 Ala, 75 Ile) and ᴬγᵀ (136 Ala, 75 Thr). CM-Sephadex chromatography can be combined to attain a further enrichment. Hb F isolated from adult blood was essentially identical with the protein from cord blood in their physicochemical and physiological properties. Developmental changes in the γ isoform composition of Hb F were studied in 110 normal Japanese newborns, 297 infants within one year after birth and 121 adults, first by the γCB-3 peptide procedure and then by HPLC method. The average ratio of ᴳγ/ᴬγ changed from 7/3 at birth to 4/6 during the postnatal 4- to 5-month period, and thereafter settled down gradually to 3/7 in the adults. The most conspicuous finding was that the isoform composition and therefore the γ gene expression, which was kept within a strictly confined level in the neonatal period, was
rendered gradually to show a highly extensive variation during postnatal development. The ᴬγᵀ gene frequencies in the newborns and the adults were estimated as 0.125 and 0.160, respectively, and the values were consistent with those previously reported for Japanese subjects, but quite different from those for Chinese and Afro-americans. The γ isoform compositions were also studied in multiple pregnancies. The results suggested the presence of an "external" factor affecting the expression of the γ globin genes and also the possibility of using the γ isoform compositions to determine the zygosity of twins, albeit under limited conditions.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.489-5020469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1865奈良医学雑誌, 43(5): 489-502http://hdl.handle.net/10564/1865http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1865/1/489-502p%ef%bc%9a%e7%94%9f%e5%be%8c%e7%99%ba%e8%82%b2%e3%81%ab%e4%bc%b4%e3%81%86%e8%83%8e%e5%85%90%e3%83%98%e3%83%a2%e3%82%b0%e3%83%ad%e3%83%93%e3%83%b3%ce%b3%e9%8e%96%e7%95%b0%e5%9e%8b%e4%bd%93%e7%b5%84%e6%88%90%e3%81%ae%e5%a4%89%e5%8c%96%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6%ef%bc%9a%e3%81%84%e3%82%8f%e3%82%86%e3%82%8bA-F_switching%e3%81%ab%e9%96%a2%e9%80%a3%e3%81%97%e3%81%a6.pdf04695550AN00180974奈良医学雑誌4354895021992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18662017-06-11T23:20:26Zhdl_10564_1858血友病Bᴍ症例のpolymerase chain reaction後の増幅DNAを用いたsingle strand conformation polymorphism法による遺伝子異常の検討ANALYSIS OF GENE ABNORMALITY IN HEMOPHILIA Bᴍ PATIENTS USING AMPLIFIED DNA WITH POLYMERASE CHAIN REACTION (PCR) AND SINGLE STRAND CONFORMATION POLYMORPHISM (SSCP) METHOD久世, 晋徳hemophilia Bᴍfactor Ⅸpolymerase chain reaction (PCR)single strand conformation polymorphism (SSCP)Gene abnormalities in 5 hemophilia Bᴍ patients were detected by amplified DNA following single strand comformation polymorphism (SSCP) method. The amplification
was performed by polymerase chain reaction (PCR) method using 8 pairs of oligonucleotide
primers covering 8 exons. The SSCP method revealed that gene abnormalities were found on exon f in factor Ⅸ Kashihara and on exon h in factor Ⅸ Amagasaki, factor Ⅸ Niigata, factor Ⅸ Kiryuu and factor Ⅸ Takatsuki. DNA sequence analysis detected a single identical G to T transition at nucleotide 20,524 in factor Ⅸ Kashihara, C to T transition at nucleotide 31,290 in factor Ⅸ Niigata, G to A transition at nucleotide 31,053 in factor Ⅸ Amagasaki, T to A transition at nucleotide 31,059 in factor Ⅸ Kiryuu and G to A transition at nucleotide 31,278 in factor Ⅸ Takatsuki.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.503-5100469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1866奈良医学雑誌, 43(5): 503-510http://hdl.handle.net/10564/1866http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1866/1/503-510p%ef%bc%9a%e8%a1%80%e5%8f%8b%e7%97%85B_M%e7%97%87%e4%be%8b%e3%81%aepolymerase_chain_reaction%e5%be%8c%e3%81%ae%e5%a2%97%e5%b9%85DNA%e3%82%92%e7%94%a8%e3%81%84%e3%81%9fsingle_strand_conformation_polymorphism%e6%b3%95%e3%81%ab%e3%82%88%e3%82%8b%e9%81%ba%e4%bc%9d%e5%ad%90%e7%95%b0%e5%b8%b8%e3%81%ae%e6%a4%9c%e8%a8%8e.pdf04695550AN00180974奈良医学雑誌4355035101992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18672017-06-11T23:20:26Zhdl_10564_1858Enzyme-Linked Immunosorbent Assayによる第Ⅷ因子抑制物質中のIgG subclassの検討ANALYSIS OF IgG SUBCLASS IN FACTOR Ⅷ INHIBITOR BY ENZYME-LINKED IMMUNOSORBENT ASSAY澤本, 好克hemophilia Afactor Ⅷ (F. Ⅷ)inhibitorIgG subclassenzyme-linked immunosorbent assay (ELISA)Semi-quantitative analysis for factor Ⅷ (F. Ⅷ)-specific IgG subclass in the plasma of hemophilia A with F. Ⅷ inhibitor was developed by enzyme-linked immunosorbent
assay (ELISA) using immobilized recombinant F. Ⅷ. The bound F. Ⅷ-specific IgG subclass was detected by monoclonal anti-IgG₁, IgG₂, IgG₃ and IgG₄ antibodies followed
by peroxidase conjugated goat anti-mouse IgG. Out of 82 plasma samples from 14 patients with inhibitor, 53 samples from 11 patients were positive for only IgG₄. There was no sample which was positive for only IgG₁, IgG₂ or IgG₃. Of the eleven patients with samples which were positive for only IgG₄, one patient was also positive for only IgG₁ in one sample, four patients were also positive for only IgG₂ in eight samples and one patient was positive
for only IgG₃ in three samples on different dates. None of F. Ⅷ-specific IgG subclass was detected in the plasma from 27 samples of hemophilia A without inhibitor. In conclusion, the ELISA system was suitable not only for the detection of F. Ⅷ inhibitor but also for semi-quantitative estimation of changes in the plasma content of F. Ⅷ-specific IgG subclass. Furthermore, it was demonstrated that the dominant subclass among the inhibitors was IgG₄. However, other IgG subclasses were also detected in some cases.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.511-5210469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1867奈良医学雑誌, 43(5): 511-521http://hdl.handle.net/10564/1867http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1867/1/511-521p%ef%bc%9aEnzyme-Linked_Immunosorbent_Assay%e3%81%ab%e3%82%88%e3%82%8b%e7%ac%ac%e2%85%a7%e5%9b%a0%e5%ad%90%e6%8a%91%e5%88%b6%e7%89%a9%e8%b3%aa%e4%b8%ad%e3%81%aeIgG_subclass%e3%81%ae%e6%a4%9c%e8%a8%8e.pdf04695550AN00180974奈良医学雑誌4355115211992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18682017-05-29T06:07:49Zhdl_10564_1858大和高原三村の食生活構造と健診情報との相関性の検討AN ASSOCIATION BETWEEN EATING HABITS AND LABORATORY DATA AMONG PEOPLE PARTICIPATING IN COMMUNITY-BASED MASS EXAMINATIONS IN YAMATO-KOGEN DISTRICT IN NARA PREFECTURE大門, 位守榎本, 康博車谷, 典男坂梨, 照子和田, 昭治森山, 忠重川口, 忠男杉本, 和夫eating habitsfood frequency questionnairemultivariate analysisTo clarify the characteristics of eating habits among the residents of Yamato-Kogen district we conducted a principal component analysis of food frequency questionnaire (consisting of 14 items) obtained from the people participating in community-based mass examinations. The examinations were performed in the district under study between July and September in 1991. Eigen values, contribution rations and loading factors were calculated from correlation matrix among 14 items. As the first principal component, the items of Q10 (food cooked with oil), Q7 (boiled fish pastes), Q5 (fishes), Q3 (eggs), Q13 (tsukudani), Q4 (meats), Q11 (miso soup) were extracted, and as the second one those of Q1 (cooked paddy rice), Q13 (tsukudani) in Q6 (salted, semidried, split fishes) and Q12 (tsukemono) were extracted. The first principal component was considered to represent
a shift from conventinal foods in the countryside, and the second one conventional
village-type foods. Principal scores of the 1st to the 3rd principal components were yielded indivisually using loading factors for each subgroup classified by sex and village. Distribution patterns of two of these components indicated that the differences were found in eating habits not only between men and women but also among the villages under study. The distribution patterns could also clearly identify the persons whose eating habits deviated nutritionally. For the same subjects a multiple regression analysis was done on all kinds of laboratory data and 14 items of food frequency questionnaire. The results revealed the existence of associations between eating habits and laboratory data.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.522-5470469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1868奈良医学雑誌, 43(5): 522-547http://hdl.handle.net/10564/1868http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1868/1/522-547p%ef%bc%9a%e5%a4%a7%e5%92%8c%e9%ab%98%e5%8e%9f%e4%b8%89%e6%9d%91%e3%81%ae%e9%a3%9f%e7%94%9f%e6%b4%bb%e6%a7%8b%e9%80%a0%e3%81%a8%e5%81%a5%e8%a8%ba%e6%83%85%e5%a0%b1%e3%81%a8%e3%81%ae%e7%9b%b8%e9%96%a2%e6%80%a7%e3%81%ae%e6%a4%9c%e8%a8%8e.pdf04695550AN00180974奈良医学雑誌4355225471992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18692017-05-29T06:07:50Zhdl_10564_1858血友病Aインヒビター患者におけるブタ第Ⅷ因子製剤(Hyate : C)の反応性REACTIVITY OF PORCINE FACTOR Ⅷ PREPARATION (HYATE : C) TO FACTOR Ⅷ INHIBITOR IN HEMOPHILIA A寺田, 茂紀hemophilia Ahuman factor Ⅷ inhibitorporcine factor Ⅷ inhibitorporcine factor Ⅷ preparation (Hyate : C)Human and porcine factor Ⅷ inhibitor titers were measured in the plasma of 15 hemophilia A patients with inhibitor and binding fragment to human factor Ⅷ polypeptide was analyzed by immunoblot method. 1. Human factor Ⅷ inhibitor levels were 4-390 B.U/ml, and porcine factor Ⅷ inhibitor levels that were 0-58 B.U/ml were lower than inhibitor levels to human factor Ⅷ. Cross
reactivity (ratio of porcine to human factor Ⅷ inhibitor titer) was 0-100%. 2. Two cases with factor Ⅷ inhibitor binding to 44 kDa fragment of human factor Ⅷ heavy chain had low crossreactivity (0.5%-0%). One case with inhibitor binding to 44 kDa fragment and 72 kDa fragment of human factor Ⅷ light chain had crossreactivity of 11%. 10 cases with inhibitor binding to 72 kDa fragment had high crossreactivity (24-100%). Two cases with inhibitor binding to 44 kDa and B domain had crossreactivity of 1% and 27%.
3. Hemostatic effect of the porcine factor Ⅷ preparation was observed in 3 cases with
factor Ⅷ inhibitor.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.548-5560469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1869奈良医学雑誌, 43(5): 548-556http://hdl.handle.net/10564/1869http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1869/1/548-556p%ef%bc%9a%e8%a1%80%e5%8f%8b%e7%97%85A%e3%82%a4%e3%83%b3%e3%83%92%e3%83%93%e3%82%bf%e3%83%bc%e6%82%a3%e8%80%85%e3%81%ab%e3%81%8a%e3%81%91%e3%82%8b%e3%83%96%e3%82%bf%e7%ac%ac%e2%85%a7%e5%9b%a0%e5%ad%90%e8%a3%bd%e5%89%a4%28Hyate%ef%bc%9aC%29%e3%81%ae%e5%8f%8d%e5%bf%9c%e6%80%a7.pdf04695550AN00180974奈良医学雑誌4355485561992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18722017-05-29T06:07:49Zhdl_10564_1858大和高原三村における住民健診受診者の血圧値および血清脂質の推移 : 1987年度から1991年度の連続受診者649名の解析CHRONOLOGICAL CHANGES IN BLOOD PRESSURE AND SERUM LIPID LEVELS AMONG PEOPLE PARTICIPATING IN COMMUNITY-BASED MASS EXAMINATIONS IN THREE VILLAGES IN THE NORTHERN PART OF NARA PREFECTURE : AN ANALYSIS OF DATA ON 649 PEOPLE FROM 1987 TO 1991榎本, 康博車谷, 典男大門, 位守坂梨, 照子和田, 昭治森山, 忠重川口, 忠男杉本, 和夫blood pressurechronological changecommunity-based mass examinationserum lipid levelChronological changes in blood pressure and serum lipid levels were investigated in the people (232 men and 417 women) participating in annual community-based mass examinations which were carried out from 1987 to 1991 in three villages in the northern part of Nara Prefecture. The results obtained are summarized as follows ; 1) Around 40% of the people participating in the mass examinations in each year attended the examinations annually from 1987 to 1991. 2) Fourteen percent of men participating in the examinations annually had medical treatment for some kind of disease, of which 47% was hypertension. The corresponding figures for women were 16% and 70% respectively. 3) Prevalence of people judged to be hypertensive at the time of mass examinations
decreased chronologically from 1987 to 1991. 4) Prevalence of the hypertensive people including under treatment for hypertension
decreased chronologically in men, but increased in women. Prevalence of women with good controlled blood pressure increased chronologically. Among the hypertensives, prevalence of the persons without any treatment was the highest among men of their 50s. 5) Mean serum total cholesterol levels of both men and women were significantly higher at the last examination than the first one. 6) Serum HDL cholesterol levels showed no significant chronological change.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.557-5650469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1872奈良医学雑誌, 43(5): 557-565http://hdl.handle.net/10564/1872http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1872/1/557-565p%ef%bc%9a%e5%a4%a7%e5%92%8c%e9%ab%98%e5%8e%9f%e4%b8%89%e6%9d%91%e3%81%ab%e3%81%8a%e3%81%91%e3%82%8b%e4%bd%8f%e6%b0%91%e5%81%a5%e8%a8%ba%e5%8f%97%e8%a8%ba%e8%80%85%e3%81%ae%e8%a1%80%e5%9c%a7%e5%80%a4%e3%81%8a%e3%82%88%e3%81%b3%e8%a1%80%e6%b8%85%e8%84%82%e8%b3%aa%e3%81%ae%e6%8e%a8%e7%a7%bb%ef%bc%9a1987%e5%b9%b4%e5%ba%a6%e3%81%8b%e3%82%891991%e5%b9%b4%e5%ba%a6%e3%81%ae%e9%80%a3%e7%b6%9a%e5%8f%97%e8%a8%ba%e8%80%85649%e5%90%8d%e3%81%ae%e8%a7%a3%e6%9e%90.pdf04695550AN00180974奈良医学雑誌4355575651992-10-31jpnpublisher
oai:ginmu.naramed-u.ac.jp:10564/18732017-05-29T06:07:49Zhdl_10564_1858旋毛虫の成虫抗原に関する研究STUDIES ON TRICHINELLA SPIRALIS ADULT ANTIGENS鈴木, 秀和Trichinella spiralisadult wormsimmunodiagnosisprotective immunityelectron microscopyA zoonotic nematoda, Trichinella spiralis, has three distinct developmental stages including muscle larva, adult, and newborn larva. The aim of this study was to define
antigenic substances in adult worms of the parasite, and to assess specificity of the antigens by means of immunoelectron microscopy, which would be essential not only for developing better immunodiagnostic antigen but also for understanding of the basic issue of protective immunity. The methods included : 1) preparation of adult worms for ultrathin sections ; 2) immunostaining with sera from patients with trichinosis to localize antigenic substances
in adult worms ; 3) immunostaining with sera from other parasite infections to assess specificity of the antigens ; and 4) observation under an electron microscope. Control experiments were performed to confirm specificity by immunostaining. Subcellular structures that were positive by immunostaining with patients' sera included
hypodermis, hemolymph, hypodermal gland, brush border, intersperm substances, exocrine
granules of male genital tract, microvilli of ova and fetus cytoplasm. cuticle inner layers were weakly positive. The surface of cuticle and stichocyte granules were negative by immunostaining. The class of the corresponding immunoglobulin was predominantly G. These adult antigens were
devoid of prominent species specificity because the above-mentioned structures were more or less immunostained by sera from other helminthiasis patients including paragonimiasis, cysticercosis, fascioliasis, trichuriasis, dirofilariasis and anisakiasis.奈良医学会ArticleDepartmental Bulletin Paperapplication/pdf奈良医学雑誌 Vol.43 No.5 p.566-5860469555013450069http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1873奈良医学雑誌, 43(5): 566-586http://hdl.handle.net/10564/1873http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/1873/1/566-586p%ef%bc%9a%e6%97%8b%e6%af%9b%e8%99%ab%e3%81%ae%e6%88%90%e8%99%ab%e6%8a%97%e5%8e%9f%e3%81%ab%e9%96%a2%e3%81%99%e3%82%8b%e7%a0%94%e7%a9%b6.pdf04695550AN00180974奈良医学雑誌4355665861992-10-31jpnpublisher